Restriction endonuclease analysis for the identification of baculovirus pesticides | Zendy

Lois K. Miller | Zendy; K P Dawes | Zendy

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Restriction endonuclease analysis for the identification of baculovirus pesticides

Author(s) -

Lois K. Miller,

K P Dawes

Publication year - 1978

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.35.2.411-421.1978

Subject(s) - autographa californica , trichoplusia , biology , dna , restriction enzyme , baculoviridae , ecori , nuclear polyhedrosis virus , spodoptera , restriction fragment length polymorphism , genetics , genome , virology , microbiology and biotechnology , pest analysis , polymerase chain reaction , noctuidae , virus , gene , recombinant dna , botany

Gel electrophoresis of deoxyribonucleic acid (DNA) fragments generated by digesting the DNA genomes of nuclear polyhedrosis viruses (NPV) with restriction endonucleases provides DNA fragment patterns that may be used to identify different viruses of this group. Characteristic fragment patterns were obtained for three NPVs, which are important as biological pesticides (Autographa californica NPV, Orgyia pseudotsugata NPV, and Heliothis zea NPV). The DNA fragment patterns of the A. californica NPV genoms did not change with passage through the alternate insect host, Trichoplusia ni. Heterogeneity in one preparation of O. pseudotsugata NPV was observed. The identification procedure is direct and precise. Applications of this procedure include quality control of commercial preparations of viral pesticides and screening for genetic alterations in the viruses.

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