Immunolatex localization by scanning electron microscopy of intestinal bacteria from cockroaches
Author(s) -
J. W. Bracke,
A. J. Markovetz
Publication year - 1978
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.35.1.166-171.1978
Subject(s) - periplaneta , cockroach , bacteria , obligate anaerobe , obligate , microbiology and biotechnology , intestinal bacteria , electron microscope , biology , fusobacterium , conjugate , microscopy , scanning electron microscope , chemistry , bacteroides , materials science , pathology , optics , medicine , physics , ecology , mathematical analysis , genetics , mathematics , composite material
An immunolatex reagent was developed from antiserum to Fusobacterium varium, an obligate anaerobe isolated from the colon of the cockroach, Periplaneta americana L. A new technique that enabled the preparation of a highly efficient immunolatex conjugate was used to localize the bacterium with scanning electron microscopy, in situ, in the mixed gut contents.
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