Open AccessMedia for identification of Gibberella zeae and production of F-2-(Zearalenone)
Author(s) -
Charles W. Bacon,
Joe D. Robbins,
James K. Porter
Publication year - 1977
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.33.2.445-449.1977
Subject(s) - gibberella zeae , zearalenone , gibberella , fusarium , yeast extract , biology , incubation , agar , bran , food science , corn steep liquor , maltose , botany , fermentation , bacteria , sucrose , biochemistry , raw material , ecology , genetics
Media are described for the isolaton of Fusarium graminearum in the perithecial state, Gibberella zeae, and for the production of F-2 (zearalenone) by Fusarium species. On soil extract-corn meal agar isolated medium, G. Zeae produced perithecia in 9 to 14 days under a 12-h photoperiod. Species of Fusarium were screened for F-2 production on a liquid medium. From strains that produced F-2, the yields, from stationary cultures of G. zeae and F. culmorum after 12 days of incubation, ranged from 22 to 86 mg/liter. Three strains produced no F-2. Glumatic acid, starch, yeast extract,and the proper ratio of medium volume-to-flask volume were necessary for F-2 synthesis.
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