Open AccessMechanism of Cooperative Degradation of Gum Arabic Arabinogalactan Protein by Bifidobacterium longum Surface Enzymes
Author(s) -
Yuki Sasaki,
Masahiro Komeno,
Akihiro Ishiwata,
Ayako Horigome,
Toshitaka Odamaki,
Jinzhong Xiao,
Katsunori Tanaka,
Yukishige Ito,
Kuninori Kitahara,
Hisashi Ashida,
Kiyotaka Fujita
Publication year - 2022
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.02187-21
Subject(s) - arabinogalactan , bifidobacterium longum , gum arabic , biochemistry , tetrasaccharide , glycoside hydrolase , oligosaccharide , enzyme , biology , bifidobacterium , lactose , galactose , polysaccharide , fermentation , chemistry , food science , lactobacillus
Terminal galactose residues of the side chain of gum arabic arabinogalactan protein (AGP) are mainly substituted by α1,3/α1,4-linked Araf and β1,6-linked α-l -Rhap -(1→4)-β-d -Glcp A residues. This study found a multidomain BlArafE with GH43_22 and GH43_34 catalytic domains showing cooperative action for degrading α1,3/α1,4-linked Araf of the side chain of gum arabic AGP.