Open AccessHigh-Level Production of Isoleucine and Fusel Alcohol by Expression of the Feedback Inhibition-Insensitive Threonine Deaminase in Saccharomyces cerevisiae
Author(s) -
Shota Isogai,
Akira Nishimura,
Atsushi Kotaka,
Naoyuki Murakami,
Natsuki Hotta,
Hiroki Ishida,
Hiroshi Takagi
Publication year - 2022
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.02130-21
Subject(s) - fusel alcohol , saccharomyces cerevisiae , threonine , isoleucine , biochemistry , chemistry , biology , alcohol , leucine , enzyme , amino acid , yeast , serine
Fruit-like flavors of isoleucine-derived volatile compounds, 2-methyl-1-butanol (2MB) and its acetate ester, contribute to a variety of the flavors and tastes of alcoholic beverages. Besides its value as aroma components in foods and cosmetics, 2MB has attracted significant attention as second-generation biofuels.