Open AccessFunctional Analysis of H + -Pumping Membrane-Bound Pyrophosphatase, ADP-Glucose Synthase, and Pyruvate Phosphate Dikinase as Pyrophosphate Sources in Clostridium thermocellum
Author(s) -
Teun Kuil,
Shuen Hon,
Johannes Yayo,
Charles Foster,
Giulia Ravagnan,
Costas D. Maranas,
Lee R. Lynd,
Daniel G. Olson,
Antonius J. A. van Maris
Publication year - 2022
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.01857-21
Subject(s) - biochemistry , inorganic pyrophosphatase , clostridium thermocellum , heterologous expression , biology , pyrophosphate , phosphofructokinase , glycolysis , enzyme , gene , recombinant dna , cellulase
Increased understanding of the central metabolism ofC. thermocellum is important from a fundamental as well as from a sustainability and industrial perspective. In addition to showing that H+ -pumping membrane-bound PPase, glycogen cycling, a Ppdk–malate shunt cycle, and acetate cycling are not significant sources of PPi supply, this study adds functional annotation of four genes and availability of an updated PPi stoichiometry from biosynthesis to the scientific domain.