Open AccessPurification and Characterization of the Isoprene Monooxygenase from Rhodococcus sp. Strain AD45
Author(s) -
Leanne P. Sims,
Colin W. J. Lockwood,
Andrew T. Crombie,
Justin M. Bradley,
Nick E. Le Brun,
J. Colin Murrell
Publication year - 2022
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.00029-22
Subject(s) - isoprene , pseudomonas putida , monooxygenase , biochemistry , rhodococcus , methane monooxygenase , oxygenase , chemistry , bacteria , escherichia coli , dioxygenase , biology , stereochemistry , enzyme , organic chemistry , gene , cytochrome p450 , copolymer , genetics , polymer
Isoprene is a highly abundant climate-active gas and a carbon source for some bacteria. Analyses of the genes encoding isoprene monooxygenase (IsoMO) indicate this enzyme is a soluble diiron center monooxygenase in the same family of oxygenases as soluble methane monooxygenase, alkene monooxygenase, and toluene monooxygenase.