Open AccessMolecular Analysis of Rifampin Resistance in Bacillus anthracis and Bacillus cereus
Author(s) -
Amy J. Vogler,
Joseph D. Busch,
Stephanie Percy-Fine,
Christine Tipton-Hunton,
Kimothy L. Smith,
Paul Keim
Publication year - 2002
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.46.2.511-513.2002
Subject(s) - bacillus anthracis , bacillus cereus , mutant , rpob , microbiology and biotechnology , biology , bacteria , rifampicin , bacillaceae , gene , bacillales , antibiotics , genetics , bacillus subtilis , 16s ribosomal rna
Rifampin-resistant mutants were selected from UV-light-treated Bacillus cereus (20 mutants) and attenuated B. anthracis (23 mutants). In addition, spontaneous rifampin-resistant mutants were also isolated in attenuated B. anthracis (22 mutants). The rifampin resistance clusters of the rpoB gene were sequenced for all 65 mutants. Mutations associated with resistance were consistent with those from other bacteria, though two novel changes were observed. The spontaneous rate of resistance was estimated at 1.57 x 10(-9) mutations/generation by a Luria-Delbrück fluctuation test.