Open AccessIdentification and Characterization of the Point Mutation Which Affects the Transcription Level of the Chromosomal 3- N -Acetyltransferase Gene of Streptomyces griseus SS-1198
Author(s) -
Jun Ishikawa,
Atsuko Sunada,
Ritsuko Oyama,
Kunimoto Hotta
Publication year - 2000
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.44.2.437-440.2000
Subject(s) - streptomyces griseus , gene , biology , mutant , genetics , acetyltransferase , point mutation , transcription (linguistics) , gene expression , microbiology and biotechnology , promoter , streptomyces , acetylation , linguistics , philosophy , bacteria
We determined the molecular basis for the enhanced expression of theaac(3)-Xa gene encoding an aminoglycoside 3-N -acetyltransferase inStreptomyces griseus . A C→T substitution was identified at the putative promoter of the mutant gene. RNA analyses demonstrated that the substitution caused a marked increase in the production of the gene-specific transcripts. Therefore, it seemed very likely that theaac(3)-Xa gene was activated by the substitution resulting in the emergence of a stronger promoter.