Open AccessGenetic Characterization of vanG , a Novel Vancomycin Resistance Locus of Enterococcus faecalis
Author(s) -
Stuart J. McKessar,
Anne M. Berry,
Jan M. Bell,
John Turnidge,
James C. Paton
Publication year - 2000
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.44.11.3224-3228.2000
Subject(s) - teicoplanin , vancomycin , locus (genetics) , enterococcus faecalis , biology , genetics , microbiology and biotechnology , enterococcus , gene , genotype , open reading frame , bacteria , peptide sequence , staphylococcus aureus , escherichia coli
Enterococcus faecalis strain WCH9 displays a moderate level of resistance to vancomycin (MIC = 16 microgram/ml) and full susceptibility to teicoplanin but is negative by PCR analysis using primers specific for all known enterococcal vancomycin resistance genotypes (vanA, vanB, vanC, vanD, and vanE). We have isolated and sequenced a novel putative vancomycin resistance locus (designated vanG), which contains seven open reading frames, from this strain. These are organized differently from those of all the other enterococcal van loci, and, furthermore, the individual vanG gene products exhibit less than 50% amino acid sequence identity to other van gene products.