Open AccessMethionine Interference in Rapamycin Production Involves Repression of Demethylrapamycin Methyltransferase and S -Adenosylmethionine Synthetase
Author(s) -
Wan-Seop Kim,
Yi Wang,
Aiqi Fang,
Arnold L. Demain
Publication year - 2000
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.44.10.2908-2910.2000
Subject(s) - methionine , methyltransferase , biosynthesis , psychological repression , methionine synthase , biochemistry , enzyme repression , transferase , biology , chemistry , enzyme , methylation , amino acid , gene , gene expression
In a chemically defined medium,l -methionine decreased production of rapamycin and increased that of demethylrapamycin. Growth withl -methionine yielded cells with a lower ability to convert demethylrapamycin to rapamycin and decreased the level ofS -adenosylmethionine synthetase andS -adenosylmethionine. Thus, methionine represses at least one methyltransferase of rapamycin biosynthesis andS -adenosylmethionine synthetase.