Genetic Characterization of Antimicrobial Resistance in Canadian Isolates of Salmonella Serovar Typhimurium DT104 | Zendy

LaiKing Ng | Zendy; Michael R. Mulvey | Zendy; Irene Martín | Zendy; Geoffrey A. Peters | Zendy; Wendy Johnson | Zendy

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Genetic Characterization of Antimicrobial Resistance in Canadian Isolates of Salmonella Serovar Typhimurium DT104

Author(s) -

LaiKing Ng,

Michael R. Mulvey,

Irene Martín,

Geoffrey A. Peters,

Wendy Johnson

Publication year - 1999

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.43.12.3018

Subject(s) - spectinomycin , biology , microbiology and biotechnology , kanamycin , salmonella , tetracycline , plasmid , serotype , chloramphenicol , ampicillin , integron , antibiotic resistance , gene , streptomycin , genetics , antibiotics , bacteria

PCR was used to identify antibiotic resistance determinants in 31 CanadianSalmonella serovar Typhimurium DT104 isolates. Genes encoding resistance to ampicillin (pse1 orblaP1 ), chloramphenicol (pasppflo -like), streptomycin-spectinomycin (aadA2 ), sulfonamide (sulI ), and tetracycline [tet (G)] were mapped to a 13-kb region of DNA of one isolate. Two copies ofsulI were identified and mapped to the 3′ end of eitherpse1 oraadA2 integrons. The two integrons were separated by thepasppflo -like gene and thetet (G) gene. The kanamycin resistance determinant (aphA-1 ) was present on a 2.0-MDa plasmid (five isolates) or on the chromosome (three isolates).

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