The Cys607→Tyr Change in the UL97 Phosphotransferase Confers Ganciclovir Resistance to Two Human Cytomegalovirus Strains Recovered from Two Immunocompromised Patients | Zendy

Fausto Baldanti | Zendy; Mark Underwood | Zendy; Christine L. Talarico | Zendy; L. Simoncini | Zendy; Antonella Sarasini | Zendy; Karen K. Biron | Zendy; Giuseppe Gerna | Zendy

Open Access

The Cys607→Tyr Change in the UL97 Phosphotransferase Confers Ganciclovir Resistance to Two Human Cytomegalovirus Strains Recovered from Two Immunocompromised Patients

Author(s) -

Fausto Baldanti,

Mark Underwood,

Christine L. Talarico,

L. Simoncini,

Antonella Sarasini,

Karen K. Biron,

Giuseppe Gerna

Publication year - 1998

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.42.2.444

Subject(s) - ganciclovir , human cytomegalovirus , phosphotransferase , virology , recombinant dna , cytomegalovirus , strain (injury) , biology , betaherpesvirinae , pep group translocation , microbiology and biotechnology , herpesviridae , virus , phosphorylation , enzyme , gene , viral disease , biochemistry , phosphoenolpyruvate carboxykinase , anatomy

Two ganciclovir (GCV)-resistant human cytomegalovirus (HCMV) strains recovered from an AIDS patient (strain VR4990) and a heart transplant recipient (strain VR5474) showed a Cys607→Tyr change in the UL97-encoded phosphotransferase. No amino acid substitutions were observed in the viral DNA polymerase. Marker transfer experiments showed marked reduction in GCV phosphorylation and drug susceptibility of the recombinant HCMV strain VR4990rec2-1-1. These results further extend the region of the carboxy-terminal domain of the UL97 phosphotransferase involved in GCV substrate recognition.

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