Nucleotide and Amino Acid Sequences of the Metallo-β-Lactamase, ImiS, from Aeromonas veronii bv. sobria | Zendy

Timothy R. Walsh | Zendy; William A. Neville | Zendy; Milan Haran | Zendy; D A Tolson | Zendy; David Payne | Zendy; John H. Bateson | Zendy; Alasdair MacGowan | Zendy; Peter M. Bennett | Zendy

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Nucleotide and Amino Acid Sequences of the Metallo-β-Lactamase, ImiS, from Aeromonas veronii bv. sobria

Author(s) -

Timothy R. Walsh,

William A. Neville,

Milan Haran,

D A Tolson,

David Payne,

John H. Bateson,

Alasdair MacGowan,

Peter M. Bennett

Publication year - 1998

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.42.2.436

Subject(s) - amino acid , biology , aeromonas veronii , aeromonas , nucleic acid sequence , peptide sequence , vibrionaceae , open reading frame , microbiology and biotechnology , biochemistry , gene , bacteria , genetics

TheAeromonas veronii bv. sobria metallo-β-lactamase gene,imiS , was cloned. TheimiS open reading frame extends for 762 bp and encodes a protein of 254 amino acids with a secreted modified protein of 227 amino acids and a predicted pI of 8.1. To confirm the predicted sequence, purified ImiS was digested and the resulting peptides were identified, yielding an identical sequence for ImiS, with 98% identity to CphA. Both possessed the putative active-site sequence Asn-Tyr-His-Thr-Asp at positions 88 to 92, which is unique to theAeromonas metallo-β-lactamases.

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