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We tested 16 erythromycin-resistant clinical isolates ofS. aureus , recovered from patients hospitalized in the United States from 1958 to 1969, for the presence ofermA ,ermB , andermC by using PCR. Fifteen of 16 isolates contained at least one copy ofermA ; the remaining isolate, which was also clindamycin resistant, containedermB . Eight of the 15 isolates harboringermA , all of which were inducible, contained a single copy of the gene in the chromosome, while the remaining seven isolates had two copies of the gene.ermB was plasmid encoded and mediated constitutive resistance to erythromycin.

Characterization of Erythromycin-Resistant Isolates of Staphylococcus aureus Recovered in the United States from 1958 through 1969