Identification and characterization of IS1476, an insertion sequence-like element that disrupts VanY function in a vancomycin-resistant Enterococcus faecium strain | Zendy

Martin MacKin | Zendy; Mike Drebot | Zendy; Gregory J. Tyrrell | Zendy

Open Access

Identification and characterization of IS1476, an insertion sequence-like element that disrupts VanY function in a vancomycin-resistant Enterococcus faecium strain

Author(s) -

Martin MacKin,

Mike Drebot,

Gregory J. Tyrrell

Publication year - 1997

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.41.8.1805

Subject(s) - enterococcus faecium , insertion sequence , microbiology and biotechnology , vancomycin , biology , strain (injury) , enterococcus , sequence analysis , antibacterial agent , gene , antibiotics , bacteria , genetics , staphylococcus aureus , transposable element , anatomy , genome

The vanY gene of vancomycin-resistant enterococci encodes a D,D-carboxypeptidase. By using a PCR detection strategy, a VanA Enterococcus faecium clinical isolate was found to have an insertion sequence (IS)-like element designated IS1476 in vanY. The activity of the VanY D,D-carboxypeptidase in this isolate was decreased in a fluorometric fluoraldehyde o-phthalaldehyde assay with diacetyl-L-Lys-D-Ala-D-Ala as the substrate. This, to our knowledge, is the first report of an IS-like element in a vancomycin resistance gene.

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