Directly repeated insertion of 9-nucleotide sequence detected in penicillin-binding protein 2B gene of penicillin-resistant Streptococcus pneumoniae | Zendy

A Yamane | Zendy; Hajime Nakano | Zendy; Yasuko Asahi | Zendy; Kimiko Ubukata | Zendy; M Konno | Zendy

Open Access

Directly repeated insertion of 9-nucleotide sequence detected in penicillin-binding protein 2B gene of penicillin-resistant Streptococcus pneumoniae

Author(s) -

A Yamane,

Hajime Nakano,

Yasuko Asahi,

Kimiko Ubukata,

M Konno

Publication year - 1996

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.40.5.1257

Subject(s) - penicillin binding proteins , penicillin , streptococcus pneumoniae , nucleic acid sequence , gene , nucleotide , microbiology and biotechnology , serine , biology , antibiotics , genetics , phosphorylation

We investigated the molecular mechanism of 50 penicillin-resistant Streptococcus pneumoniae strains (penicillin: MIC, > or = 0.125 microgram/ml) having neither class A nor class B mutations in the penicillin-binding protein 2B gene (pbp2b). An analysis of the nucleotide sequences of the pbp2b genes from seven strains revealed an unique direct repeat of 9 nucleotides (TGGTATACT) between active-site serine (residue 385) and Ser-X-Asn (residues 442 to 444) motifs. The same insertion was detected in 13 strains.

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