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Oligonucleotide probes for detection of cephalosporinases among Bacteroides strains
Author(s) -
Paola Mastrantonio,
Rita Cardines,
Patrizia Spigaglia
Publication year - 1996
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.40.4.1014
Subject(s) - bacteroides fragilis , oligomer restriction , oligonucleotide , microbiology and biotechnology , bacteroides , biology , gene , beta lactamase , molecular probe , enzyme , bacteroidaceae , bacteria , escherichia coli , antibiotics , genetics , biochemistry
Two oligonucleotide probes selected from the sequences of cepA and cfxA genes, respectively, were used to detect beta-lactamase production among strains of the Bacteroides fragilis group. By using these probes, colony hybridization was shown to be a specific and rapid method for identifying the more prevalent beta-lactamase, CepA, and the rarer CfxA enzyme among B. fragilis strains.

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