Open AccessDetection of amphotericin B-resistant Candida isolates in a broth-based system
Author(s) -
John H. Rex,
Chester R. Cooper,
William G. Merz,
John N. Galgiani,
Elias Anaissie
Publication year - 1995
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.39.4.906
Subject(s) - amphotericin b , microbiology and biotechnology , antibiotics , candida infections , biology , antifungal
Because of the limited ability of the National Committee for Clinical Laboratory Standards proposed M27P methodology to detect resistance to amphotericin B by Candida isolates, we sought to identify alternative media and pH conditions that could reliably identify resistant isolates. Antibiotic Medium 3 broth (also known as Penassay broth) buffered to pH 5 or pH 7 produced superior results and readily identified a series of resistant isolates.