Open AccessnfxC-type quinolone resistance in a clinical isolate of Pseudomonas aeruginosa
Author(s) -
Hideyuki Fukuda,
Mamoru Hosaka,
Shizuko Iyobe,
Naomasa Gotoh,
Takeshi Nishino,
Keiji Hirai
Publication year - 1995
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.39.3.790
Subject(s) - norfloxacin , pseudomonas aeruginosa , imipenem , microbiology and biotechnology , chloramphenicol , quinolone , biology , gel electrophoresis , pseudomonadaceae , polyacrylamide gel electrophoresis , antibacterial agent , pseudomonadales , bacterial outer membrane , bacteria , antibiotics , chemistry , escherichia coli , gene , biochemistry , ciprofloxacin , genetics , enzyme
Quinolone resistance gene nqr-T91 in a clinical isolate of Pseudomonas aeruginosa P1481 was cotransducible with catA1 in P. aeruginosa PAO. The nqr-T91 transductant, PKH-T91, was resistant to norfloxacin, imipenem, and chloramphenicol and showed less norfloxacin accumulation than the parent strain did. Loss of the 46-kDa outer membrane protein (D2) and an increase in the 50-kDa outer membrane protein in PKH-T91 were observed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Lipopolysaccharides in the transductant were also changed. These alterations were considered to be related to lower levels of norfloxacin accumulation in PKH-T91. These genetic and biochemical properties suggested that an nfxC type of quinolone-resistant mutation occurred in a clinical isolate of P. aeruginosa P1481.