Open AccessPig kidney (LLC-PK1) cell membrane fluidity during exposure to gentamicin or tobramycin
Author(s) -
Sue J. Kohlhepp,
Linlin Hou,
David N. Gilbert
Publication year - 1994
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.38.9.2169
Subject(s) - tobramycin , aminoglycoside , gentamicin , chemistry , membrane fluidity , biophysics , membrane , antibiotics , biochemistry , biology
The surface membrane properties of LLC-PK1 cells grown with and without various amounts of gentamicin or tobramycin for various lengths of time were determined by measuring the diffusion coefficient of N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)dipalmitoyl-L- alpha-phosphatidylethanolamine (NBD-PE) and the percentage of NBD-PE free to diffuse after photobleaching. One hour of exposure to tobramycin decreased the percentage that was free to diffuse. After 1 day or longer of exposure to either aminoglycoside the percentage that was free to diffuse returned to preexposure levels and the diffusion coefficient decreased.