Open AccessTransferability and genetic relatedness of high-level gentamicin resistance among enterococci
Author(s) -
D F Sahm,
Michael S. Gilmore
Publication year - 1994
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.38.5.1194
Subject(s) - gentamicin , microbiology and biotechnology , biology , enterococcus , enterococcus faecalis , enterococcus faecium , genetics , gene , escherichia coli , antibiotics
Gentamicin resistance in six enterococcal species was investigated. Transfer of resistance was observed for the donors E. faecium UC 79, E. avium CC 54, and E. gallinarum B 51, but not for E. raffinosus UC 78 or E. casseliflavus UC 73. Except for E. casseliflavus UC 73, homology was observed between the E. faecalis aac6'-aph2" gene and DNA from other species. Whereas 2.6-kb HindIII fragments encoded resistance in E. faecalis UC 244, its transconjugant, and E. raffinosus UC 78, 3.4-kb fragments encoded resistance in E. faecium UC 79, E. gallinarum B 51, and their transconjugants. A 3.4-kb fragment encoded resistance in E. avium CC 54, but 2.6-kb fragments encoded resistance in the transconjugants. Although many similarities were found among the strains, the heterogeneity in gentamicin resistance exhibited by some isolates indicates diversity among these determinants.