Open AccessPseudomonas aeruginosa biofilm as a diffusion barrier to piperacillin
Author(s) -
B. Hoyle,
Joenel Alcantara,
J. W. Costerton
Publication year - 1992
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.36.9.2054
Subject(s) - pseudomonas aeruginosa , biofilm , piperacillin , microbiology and biotechnology , chemistry , pseudomonadaceae , diffusion , pseudomonadales , dialysis , membrane , bacteria , biology , biochemistry , medicine , surgery , genetics , physics , thermodynamics
Pseudomonas aeruginosa 579 biofilms formed on dialysis membranes retarded piperacillin diffusion. Treatment of biofilms with 5.0 mM CaCl2.2H2O prevented diffusion. Biofilms permitted equilibration of [14C]glucose. Thin-layer chromatography of fluids distal to untreated (viable and nonviable) and viable Ca(2+)-treated P. aeruginosa 579 biofilms and fluids distal to a viable P. aeruginosa mutant noninducible for the expression of beta-lactamase did not detect piperacillinoic acid.