Open AccessNucleotide sequence of the protein D2 gene of Pseudomonas aeruginosa
Author(s) -
Hiroshi Yoneyama,
Eisaku Yoshihara,
Taiji Nakae
Publication year - 1992
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.36.8.1791
Subject(s) - gene , amino acid , peptide sequence , nucleic acid sequence , pseudomonas aeruginosa , signal peptide , biology , sequence (biology) , cysteine , glycine , biochemistry , porin , chemistry , microbiology and biotechnology , escherichia coli , bacterial outer membrane , bacteria , genetics , enzyme
Protein D2 of the outer membrane of Pseudomonas aeruginosa was shown to form the imipenem-permeable pore. We cloned and sequenced the protein D2 gene. The protein D2 gene encodes a polypeptide with 443 amino acids consisting of 23 and 420 amino acid residues for the signal peptide and mature polypeptide (M(r), 46,010), respectively. Protein D2 contains the highest molar ratio of glycine and no cysteine. The polar amino acids are scattered throughout the sequence.