Measurement of the concentration of amphotericin B in brain tissue of scrapie-infected hamsters with a simple and sensitive method | Zendy

Patrizia Casaccia | Zendy; Anna Ladogana | Zendy; You Geng Xi | Zendy; Loredana Ingrosso | Zendy; Maurizio Pocchiari | Zendy; Maria Chiara Silvestrini | Zendy; Achille Cittadini | Zendy

Open Access

Measurement of the concentration of amphotericin B in brain tissue of scrapie-infected hamsters with a simple and sensitive method

Author(s) -

Patrizia Casaccia,

Anna Ladogana,

You Geng Xi,

Loredana Ingrosso,

Maurizio Pocchiari,

Maria Chiara Silvestrini,

Achille Cittadini

Publication year - 1991

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.35.7.1486

Subject(s) - amphotericin b , scrapie , spleen , incubation period , brain tissue , incubation , absorption (acoustics) , biology , chemistry , pharmacology , microbiology and biotechnology , medicine , immunology , antifungal , biochemistry , materials science , prion protein , disease , composite material

A simple, sensitive, and reproducible assay for the measurement of the amphotericin B concentration in tissue extracts was developed by using the fourth derivative of the absorption spectrum of amphotericin B between wavelengths of 330 and 430 nm. The amphotericin B concentration in spleen and brain was proportional to the total amount administered. The amphotericin B concentration in the brain was highly correlated with the increase in the mean incubation period of intracerebrally scrapie-infected hamsters.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research