Open AccessMeasurement of the concentration of amphotericin B in brain tissue of scrapie-infected hamsters with a simple and sensitive method
Author(s) -
Patrizia Casaccia,
Anna Ladogana,
You Geng Xi,
Loredana Ingrosso,
Maurizio Pocchiari,
Maria Chiara Silvestrini,
Achille Cittadini
Publication year - 1991
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.35.7.1486
Subject(s) - amphotericin b , scrapie , incubation period , spleen , brain tissue , incubation , biology , chemistry , virology , microbiology and biotechnology , medicine , immunology , antifungal , biochemistry , prion protein , disease
A simple, sensitive, and reproducible assay for the measurement of the amphotericin B concentration in tissue extracts was developed by using the fourth derivative of the absorption spectrum of amphotericin B between wavelengths of 330 and 430 nm. The amphotericin B concentration in spleen and brain was proportional to the total amount administered. The amphotericin B concentration in the brain was highly correlated with the increase in the mean incubation period of intracerebrally scrapie-infected hamsters.