Open AccessNonradioactive DNA probe for detection of gene for methicillin resistance in Staphylococcus aureus
Author(s) -
Marco Ligozzi,
G. M. Rossolini,
Elena Tonin,
Roberta Fontana
Publication year - 1991
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.35.3.575
Subject(s) - staphylococcus aureus , microbiology and biotechnology , biology , dna , micrococcaceae , gene , methicillin resistant staphylococcus aureus , antibacterial agent , antibiotics , genetics , bacteria
A DNA probe was developed by inserting, in the SmaI site of pBluescript sK, a 528-bp fragment of the gene responsible for intrinsic resistance to beta-lactam antibiotics in Staphylococcus aureus (mec determinant). The mec probe provided a useful tool for the rapid identification of the intrinsic resistance trait and for establishing guidelines for testing the in vitro susceptibility of S. aureus to beta-lactams.