Nonradioactive DNA probe for detection of gene for methicillin resistance in Staphylococcus aureus | Zendy

Marco Ligozzi | Zendy; Gian María Rossolini | Zendy; Elena Tonin | Zendy; Roberta Fontana | Zendy

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Nonradioactive DNA probe for detection of gene for methicillin resistance in Staphylococcus aureus

Author(s) -

Marco Ligozzi,

Gian María Rossolini,

Elena Tonin,

Roberta Fontana

Publication year - 1991

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.35.3.575

Subject(s) - staphylococcus aureus , microbiology and biotechnology , biology , gene , dna , micrococcaceae , antibacterial agent , antibiotics , genetics , bacteria

A DNA probe was developed by inserting, in the SmaI site of pBluescript sK, a 528-bp fragment of the gene responsible for intrinsic resistance to beta-lactam antibiotics in Staphylococcus aureus (mec determinant). The mec probe provided a useful tool for the rapid identification of the intrinsic resistance trait and for establishing guidelines for testing the in vitro susceptibility of S. aureus to beta-lactams.

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