Open AccessMechanism of action of sparfloxacin against and mechanism of resistance in gram-negative and gram-positive bacteria
Author(s) -
Laura J. V. Piddock,
Mengtao Zhu
Publication year - 1991
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.35.11.2423
Subject(s) - sparfloxacin , microbiology and biotechnology , enterobacteriaceae , escherichia coli , ciprofloxacin , pseudomonas aeruginosa , bacteria , antibacterial agent , staphylococcus aureus , gram negative bacteria , biology , minimum inhibitory concentration , chemistry , antibiotics , ofloxacin , biochemistry , genetics , gene
The inhibition of DNA synthesis by sparfloxacin; accumulation of sparfloxacin into members of the family Enterobacteriaceae, Pseudomonas aeruginosa, and staphylococci; induction of recA in Escherichia coli; and the optimum bactericidal concentration (OBC) were measured, and killing kinetics at the OBC were estimated. The OBC and maximum recA-inducing concentration in E. coli were both 1 microgram of sparfloxacin per ml. Accumulation was rapid; two- to threefold more sparfloxacin than ciprofloxacin accumulated in staphylococci and more sparfloxacin accumulated in staphylococci than in gram-negative bacteria. Laboratory mutants with decreased susceptibilities to quinolones alone or multiply resistant were selected from the Enterobacteriaceae and Staphylococcus aureus by using sparfloxacin.