Open AccessCorrelation between aminoglycoside resistance profiles and DNA hybridization of clinical isolates
Author(s) -
Karen Joy Shaw,
R S Hare,
F. Sabatelli,
M F Rizzo,
C A Cramer,
L. Naples,
S Kocsi,
H. Munayyer,
Paul A. Mann,
George H. Miller
Publication year - 1991
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.35.11.2253
Subject(s) - aminoglycoside , biology , dna–dna hybridization , gene , genetics , dna , southern blot , hybridization probe , nucleic acid thermodynamics , microbiology and biotechnology , antibiotics , rna
DNA hybridization data and aminoglycoside resistance profiles (AGRPs) were determined for 4,088 clinical isolates from three studies (United States, Belgium, and Argentina). The correlation between susceptibility profiles and hybridization results was determined with nine DNA probes. For each of the seven aminoglycoside resistance profiles which we were able to test, the data suggested at least two distinct genes could encode enzymes which lead to identical resistance profiles. Furthermore, the DNA hybridization data showed that individual strains carried up to six unique aminoglycoside resistance genes. DNA hybridization revealed interesting differences in the frequencies of these genes by organism and by country.