Open AccessNovel method for measuring growth of Treponema hyodysenteriae and its application for monitoring susceptibility of clinical isolates to antimicrobial agents
Author(s) -
Frederick Weber,
D L Earley
Publication year - 1991
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.35.10.2012
Subject(s) - treponema , broth microdilution , microbiology and biotechnology , antibiotics , hemolysis , pathogen , biology , antimicrobial , agar dilution , agar plate , bacteria , virology , minimum inhibitory concentration , immunology , syphilis , genetics , human immunodeficiency virus (hiv)
A microdilution assay method was developed to determine MICs of antibiotics for Treponema hyodysenteriae and to estimate the bacterial burden of mice colonized by this swine pathogen. Hemolysis of bovine erythrocytes in broth was correlated with growth of the spirochete, and growth inhibition by drugs could be determined by measuring hemolysis. MICs of 10 antibiotics for eight clinical isolates of T. hyodysenteriae measured by this method were similar to values obtained by an agar dilution method and to values reported in the literature. This method was also used to demonstrate the elimination of T. hyodysenteriae from the ceca of infected mice following oral treatment with carbadox. The procedure described in this paper provides a rapid and simple method for determining MICs of antibiotics for T. hyodysenteriae and may be adapted for the purpose of determining the presence of this veterinary pathogen in cecal contents of experimentally infected mice.