Effects of thymidine and uridine on the phosphorylation of 3'-azido-3'-deoxythymidine (zidovudine) in human mononuclear cells | Zendy

János Szebeni | Zendy; S S Patel | Zendy; Kenneth E. Hung | Zendy; L M Wahl | Zendy; John N. Weinstein | Zendy

Open Access

Effects of thymidine and uridine on the phosphorylation of 3'-azido-3'-deoxythymidine (zidovudine) in human mononuclear cells

Author(s) -

János Szebeni,

S S Patel,

Kenneth E. Hung,

L M Wahl,

John N. Weinstein

Publication year - 1991

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.35.1.198

Subject(s) - zidovudine , thymidine , uridine , phosphorylation , peripheral blood mononuclear cell , nucleoside , thymidine kinase , biology , chemistry , in vitro , biochemistry , virology , microbiology and biotechnology , virus , rna , viral disease , gene , herpes simplex virus

The effects of thymidine and uridine on the phosphorylation of 3'-azido-3'-deoxythymidine (AZT) were studied in various human mononuclear cell preparations. Thymidine suppressed [3H]AZT phosphorylation in the same concentration range (20 to 100 microM) in which it antagonizes the anti-human immunodeficiency virus activity of AZT. Uridine, in turn, had no influence on AZT phosphorylation, just as it has no effect on the anti-human immunodeficiency virus activity of AZT. These findings are consistent with a close relationship between the inhibition of AZT phosphorylation and the influence of physiological nucleosides on the antiviral activity of AZT.

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