Open AccessEffects of thymidine and uridine on the phosphorylation of 3'-azido-3'-deoxythymidine (zidovudine) in human mononuclear cells
Author(s) -
János Szebeni,
S. S. Patel,
Kenneth E. Hung,
Larry M. Wahl,
John N. Weinstein
Publication year - 1991
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.35.1.198
Subject(s) - zidovudine , thymidine , uridine , phosphorylation , peripheral blood mononuclear cell , nucleoside , biology , thymidine kinase , in vitro , biochemistry , microbiology and biotechnology , virology , virus , chemistry , rna , viral disease , gene , herpes simplex virus
The effects of thymidine and uridine on the phosphorylation of 3'-azido-3'-deoxythymidine (AZT) were studied in various human mononuclear cell preparations. Thymidine suppressed [3H]AZT phosphorylation in the same concentration range (20 to 100 microM) in which it antagonizes the anti-human immunodeficiency virus activity of AZT. Uridine, in turn, had no influence on AZT phosphorylation, just as it has no effect on the anti-human immunodeficiency virus activity of AZT. These findings are consistent with a close relationship between the inhibition of AZT phosphorylation and the influence of physiological nucleosides on the antiviral activity of AZT.