Open AccessNew norfloxacin resistance gene in Pseudomonas aeruginosa PAO
Author(s) -
Hideyuki Fukuda,
Mamoru Hosaka,
Keiji Hirai,
Shizuko Iyobe
Publication year - 1990
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.34.9.1757
Subject(s) - norfloxacin , mutant , microbiology and biotechnology , imipenem , pseudomonas aeruginosa , aminoglycoside , antibacterial agent , bacterial outer membrane , biology , chloramphenicol , chemistry , escherichia coli , antibiotics , bacteria , gene , biochemistry , antibiotic resistance , genetics , ciprofloxacin
A new type of norfloxacin-resistant mutant of Pseudomonas aeruginosa PAO was isolated. This mutant showed cross resistance to imipenem and chloramphenicol and hypersusceptibility to beta-lactam and aminoglycoside antibiotics. The new norfloxacin resistance gene nfxC was mapped near catA (46 min) on the PAO chromosome. Norfloxacin accumulation was decreased in the nfxC mutant; furthermore, the rate of imipenem diffusion through the outer membrane of the nfxC mutant was lower than that of the parent strain. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of outer membrane proteins showed a decrease of a 46-kilodalton protein and an increase of a 50-kilodalton protein in the nfxC mutant. We conclude the nfxC is a new norfloxacin resistance gene that affects outer membrane permeability to quinolones and other antimicrobial agents.