Open AccessActivities of (-)-carbovir and 3'-azido-3'-deoxythymidine against human immunodeficiency virus in vitro
Author(s) -
S G Carter,
Joseph Kessler,
C D Rankin
Publication year - 1990
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.34.6.1297
Subject(s) - in vitro , reverse transcriptase , virus , virology , biological activity , biology , cytotoxicity , thymidine , viral replication , nucleotidyltransferase , reverse transcriptase inhibitor , chemistry , microbiology and biotechnology , viral disease , rna , biochemistry , sida , gene
(-)-Carbovir, the minus optical isomer of carbocyclic-2',3'-didehydro-2',3'-dideoxyguanosine, has been shown to be the biologically active form for the inhibition of human immunodeficiency virus type 1 replication. The concentration of (-)-carbovir required to reduce reverse transcriptase activity by 50% compared with the control was 0.8 microM in H9 cells infected with the HTLV-IIIb strain; the 50% inhibitory concentration for cytotoxicity was greater than 2 mM in these cells. The effect of the timing of drug addition, pre- and postinfection, and the effect of increasing amounts of virus on the antiviral activities of (-)-carbovir and 3'-azido-3'-deoxythymidine were determined.