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Nucleotide sequence and intracellular location of the product of the fosfomycin resistance gene from transposon Tn2921
Author(s) -
Jesús Navas,
Javier León,
Miguel Arroyo,
Jorge M. Lobo
Publication year - 1990
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.34.10.2016
Subject(s) - transposable element , fosfomycin , open reading frame , nucleic acid sequence , biology , genetics , gene , gene product , dna , sequence (biology) , microbiology and biotechnology , peptide sequence , gene expression , genome , antibiotics
An 863-base-pair DNA fragment containing the fosfomycin resistance gene from transposon Tn2921 was sequenced. Analysis of the sequence revealed the presence of a single open reading frame that encoded the FOS polypeptide of 16 kilodaltons from Tn2921. Minicell fractionation studies showed that the FOS protein was located in the bacterial cytoplasm.

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