Open AccessgyrA and gyrB mutations in quinolone-resistant strains of Escherichia coli
Author(s) -
Shinichi Nakamura,
Mami Nakamura,
Tsuyoshi Kojima,
Hiroaki Yoshida
Publication year - 1989
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.33.2.254
Subject(s) - escherichia coli , quinolone , dna gyrase , biology , mutation , genetics , mutant , microbiology and biotechnology , gene , strain (injury) , antibiotics , anatomy
The proportion of gyrA and gyrB mutations in quinolone-resistant Escherichia coli strains was examined by introducing cloned wild-type gyrA and gyrB genes. In 25 spontaneous mutants of strain KL16, 13 had gyrA and 12 had gyrB mutations. In eight clinical isolates, five had gyrA mutations and one had a gyrB mutation; mutations in two isolates remained unidentified.