Open AccessComposite IS1-tetracycline resistance elements in aerobactin-encoding FIme plasmids from epidemic Salmonella wien
Author(s) -
Maria Assunta Casalino,
Mauro Nicoletti,
Nikolaj Junakovic,
Francesco Maimone
Publication year - 1988
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.32.8.1262
Subject(s) - plasmid , transposable element , aerobactin , biology , genetics , tetracycline , insertion sequence , salmonella , enterobacteriaceae , gene , mutant , escherichia coli , bacteria , antibiotics
Class B tetracycline resistance determinants have been identified in two aerobactin-encoding FIme plasmids representative of those isolated from epidemic Salmonella wien. Genetic data, restriction enzyme analysis of recombinant and mutant plasmids, and Southern blot hybridizations indicate that in both plasmids the class B determinant so far found and described only on Tn10-like transposons is part of a different genetic element. This composite insertion sequence element is about 7 kilobases long and has copies of insertion sequence IS1 at the ends.