Open AccessEffect of aminoglycoside concentration on reaction rates of aminoglycoside-modifying enzymes
Author(s) -
G.P.A. Bongaerts,
J. S. Vliegenthart
Publication year - 1988
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.32.5.740
Subject(s) - aminoglycoside , substrate (aquarium) , enzyme , saturation (graph theory) , chemistry , enzyme kinetics , biochemistry , antibiotics , biology , mathematics , active site , ecology , combinatorics
Reaction rates of several reference aminoglycoside-modifying enzymes were studied at various substrate concentrations. The resulting concentration-response curves showed wide variation in threshold concentration, in curve slope, in enzyme saturation, and in substrate inhibition. Together, the curves of a defined aminoglycoside panel yielded more specific information for each individual aminoglycoside-modifying enzyme tested than did conventional substrate profiles obtained at a single substrate concentration.