Open AccessRapid isoelectric focusing of plasmid-mediated beta-lactamases with Pharmacia PhastSystem
Author(s) -
S Huovinen
Publication year - 1988
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.32.11.1730
Subject(s) - isoelectric focusing , plasmid , beta (programming language) , sonication , microbiology and biotechnology , isoelectric point , antibacterial agent , biology , chromatography , chemistry , biochemistry , antibiotics , gene , enzyme , computer science , programming language
A modified isoelectric focusing method for rapid semiquantitative identification of plasmid-mediated beta-lactamases by use of the Pharmacia PhastSystem (Uppsala, Sweden) is described. Sonication of bacterial colonies collected directly from growth plates decreased the time required for the procedure. With sonic extracts of known beta-lactamase-producing strains used as controls, the assay could be completed in less than 2 h.