Open AccessInhibition of DNA gyrase by optically active ofloxacin
Author(s) -
Masako Imamura,
S Shibamura,
Isao Hayakawa,
Yasuaki Osada
Publication year - 1987
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.31.2.325
Subject(s) - dna gyrase , ofloxacin , norfloxacin , dna supercoil , ciprofloxacin , escherichia coli , pefloxacin , chemistry , microbiology and biotechnology , nalidixic acid , antibacterial agent , micrococcus luteus , novobiocin , minimum inhibitory concentration , fleroxacin , biology , dna , biochemistry , antibiotics , dna replication , gene
Inhibition of DNA gyrase activity by optically active ofloxacins was studied and compared with the inhibition of norfloxacin and ciprofloxacin. The (-)-isomer of ofloxacin inhibited the supercoiling activity of gyrase from Micrococcus luteus more effectively than did the (+)-isomer. The 50% inhibitory concentrations of (-)-, (+/-)-, and (+)-ofloxacin; norfloxacin; and ciprofloxacin for gyrase from Escherichia coli were 0.78, 0.98, 7.24, 0.78, and 1.15 microgram/ml, respectively. These values correlated well with the antibacterial activity of each compound against intact bacterial cells.