Open AccessCharacterization of beta-lactamases in situ on polyacrylamide gels
Author(s) -
Christine C. Sanders,
William E. Sanders,
Ellen Smith Moland
Publication year - 1986
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.30.6.951
Subject(s) - isoelectric focusing , polyacrylamide , beta (programming language) , bacteria , polyacrylamide gel electrophoresis , chemistry , beta lactamase inhibitors , biochemistry , antibacterial agent , microbiology and biotechnology , enzyme , biology , antibiotics , genetics , computer science , programming language
An inhibitor-based characterization system which allowed the identification of beta-lactamases after isoelectric focusing on polyacrylamide gels was developed. This system, using potassium clavulanate and oxacillin, distinguished type I chromosomally mediated enzymes from other beta-lactamases of gram-negative bacteria.