Characterization of beta-lactamases in situ on polyacrylamide gels | Zendy

Christine C. Sanders | Zendy; William E. Sanders | Zendy; Ellen Smith Moland | Zendy

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Characterization of beta-lactamases in situ on polyacrylamide gels

Author(s) -

Christine C. Sanders,

William E. Sanders,

Ellen Smith Moland

Publication year - 1986

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.30.6.951

Subject(s) - isoelectric focusing , polyacrylamide , beta (programming language) , bacteria , polyacrylamide gel electrophoresis , chemistry , beta lactamase inhibitors , biochemistry , antibacterial agent , microbiology and biotechnology , enzyme , biology , antibiotics , genetics , computer science , programming language

An inhibitor-based characterization system which allowed the identification of beta-lactamases after isoelectric focusing on polyacrylamide gels was developed. This system, using potassium clavulanate and oxacillin, distinguished type I chromosomally mediated enzymes from other beta-lactamases of gram-negative bacteria.

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