Purification and properties of DNA gyrase from a fluoroquinolone-resistant strain of Escherichia coli | Zendy

Kenichi Sato | Zendy; Yasushi Inoue | Zendy; Tatsuya Fujii | Zendy; Hiroshi Aoyama | Zendy; Manabu Inoue | Zendy; S Mitsuhashi | Zendy

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Purification and properties of DNA gyrase from a fluoroquinolone-resistant strain of Escherichia coli

Author(s) -

Kenichi Sato,

Yasushi Inoue,

Tatsuya Fujii,

Hiroshi Aoyama,

Manabu Inoue,

S Mitsuhashi

Publication year - 1986

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.30.5.777

Subject(s) - dna gyrase , escherichia coli , dna supercoil , ofloxacin , microbiology and biotechnology , strain (injury) , protein subunit , dna , enterobacteriaceae , plasmid , biology , bacteria , chemistry , ciprofloxacin , antibiotics , biochemistry , genetics , gene , dna replication , anatomy

Subunit A and B proteins of DNA gyrase were separately purified from fluoroquinolone-resistant Escherichia coli GN14181 (MIC of ofloxacin, 100 micrograms/ml) and susceptible strain KL-16. The supercoiling activities of reconstituted Ar+Br (r, resistant) and Ar+Bs (s, susceptible) were 250-fold more resistant to new fluoroquinolones than those of As+Bs and As+Br.

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