Open AccessPurification and properties of DNA gyrase from a fluoroquinolone-resistant strain of Escherichia coli
Author(s) -
Kenichi Sato,
Yasushi Inoue,
Tatsuya Fujii,
Hiroshi Aoyama,
Matsuhisa Inoue,
Susumu Mitsuhashi
Publication year - 1986
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.30.5.777
Subject(s) - dna gyrase , escherichia coli , dna supercoil , ofloxacin , microbiology and biotechnology , strain (injury) , protein subunit , dna , enterobacteriaceae , plasmid , biology , bacteria , chemistry , ciprofloxacin , antibiotics , biochemistry , genetics , gene , dna replication , anatomy
Subunit A and B proteins of DNA gyrase were separately purified from fluoroquinolone-resistant Escherichia coli GN14181 (MIC of ofloxacin, 100 micrograms/ml) and susceptible strain KL-16. The supercoiling activities of reconstituted Ar+Br (r, resistant) and Ar+Bs (s, susceptible) were 250-fold more resistant to new fluoroquinolones than those of As+Bs and As+Br.