Open AccessPlasmid penicillin resistance in Vibrio cholerae: identification of new beta-lactamase SAR-1
Author(s) -
Alison J. Reid,
S. G. B. Amyes
Publication year - 1986
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.30.2.245
Subject(s) - vibrio cholerae , plasmid , microbiology and biotechnology , penicillin , biology , vibrionaceae , carbenicillin , beta lactamase , el tor , bacteria , gene , escherichia coli , antibiotics , ampicillin , genetics
Two strains of Vibrio cholerae biotype El Tor, isolated in Tanzania, possessed a single IncC resistance plasmid of 113 kilobases. Both plasmids encoded the production of a novel beta-lactamase, SAR-1, which was 33,700 daltons in size and was able to hydrolyze carbenicillin as well as penicillin G. The SAR-1 beta-lactamase was quite distinct from all other plasmid beta-lactamases by virtue of its unusually low isoelectric point and a combination of its size, substrate profile, and inhibition properties. This enzyme is only the second beta-lactamase identified in V. cholerae species and the first to be reported in V. cholerae strains isolated in Southern Africa.