Open AccessSelective inhibition of the accumulation of extracellular proteases of Pseudomonas aeruginosa by gentamicin and tobramycin
Author(s) -
Roseanne Warren,
Neil R. Baker,
Jarrod W. Johnson,
Mark Stapleton
Publication year - 1985
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.27.4.468
Subject(s) - proteases , tobramycin , pseudomonas aeruginosa , aminoglycoside , microbiology and biotechnology , lysis , protease , secretion , extracellular , gentamicin , chemistry , biochemistry , biology , enzyme , antibiotics , bacteria , genetics
Gentamicin and tobramycin inhibited the accumulation of extracellular proteases secreted by Pseudomonas aeruginosa. The secretion of protease was inhibited at concentrations of these drugs that were below the level required to inhibit general protein synthesis. Neither magnesium ions nor high ionic strength antagonized the ability of the aminoglycosides to block secretion of the proteases. Under these culture conditions magnesium ions were shown to antagonize the effects of the aminoglycosides on protein synthesis and aminoglycoside-mediated lysozyme lysis of P. aeruginosa. These results suggested that the drugs blocked secretion of the proteases by acting at the level of the outer membrane.