Open AccessEstablishment of MICs of moxalactam for control and reference anaerobic organisms in agar dilution and microdilution techniques
Author(s) -
Vera L. Sutter,
Janet Emmerman,
Eileen L. Randall,
R.J. Zabransky,
Robert J. Birk
Publication year - 1985
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.27.3.424
Subject(s) - broth microdilution , agar dilution , bacteroides fragilis , microbiology and biotechnology , biology , agar , agar dilution method , anaerobic bacteria , minimum inhibitory concentration , bacteria , antimicrobial , antibiotics , genetics
The MICs of moxalactam were determined for eight National Committee for Clinical Laboratory Standards control and reference strains and for Fusobacterium nucleatum ATCC 10953 by agar and microdilution techniques. The recommended MIC for the control strain Bacteroides fragilis in both agar and microdilution tests is 0.5 micrograms/ml. Recommended MICs for Bacteroides thetaiotaomicron ATCC 29741 and Clostridium perfringens ATCC 13124 by agar dilution are 8 and 0.063 micrograms/ml, respectively. These two strains gave inconsistent results in microdilution tests. Variation in results with microdilution procedures was seen, which illustrates problems in reading endpoints and with modifications of media. Recommended MICs for the reference strains are presented.