Open AccessImproved micromethod for mezlocillin quantitation in serum and urine by high-pressure liquid chromatography
Author(s) -
Donatella Fiore,
François A. Auger,
George L. Drusano,
V R Dandu,
L. J. Lesko
Publication year - 1984
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.26.5.775
Subject(s) - chromatography , urine , chemistry , mezlocillin , high performance liquid chromatography , extraction (chemistry) , quantitative analysis (chemistry) , biochemistry , biology , bacteria , pseudomonas aeruginosa , genetics , piperacillin
A rapid, sensitive, and specific method of analysis for mezlocillin in serum and urine by high-pressure liquid chromatography is described. A solid-phase extraction column was used to remove interfering substances from samples before chromatography. Quantitation included the use of an internal standard, nafcillin. Mezlocillin was chromatographed with a phosphate buffer-acetonitrile (73:27) mobile phase and a C-18 reverse-phase column and detected at a wavelength of 220 nm. The assay had a sensitivity of 1.6 micrograms/ml and a linearity of up to 600 micrograms/ml and 16 mg/ml in serum and urine, respectively, with only 0.1 ml of sample. The interday and intraday coefficients of variation for replicate analyses of spiked serum and urine specimens were less than 6.5%.