Open AccessIdentification of aminoglycoside-acetylating enzymes by high-pressure liquid chromatographic determination of their reaction products
Author(s) -
A. M. Lovering,
Leroy White,
D. S. Reeves
Publication year - 1984
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.26.1.10
Subject(s) - sisomicin , netilmicin , chromatography , tobramycin , chemistry , aminoglycoside , enzyme , neomycin , kanamycin , high performance liquid chromatography , biochemistry , antibiotics , gentamicin
A method to identify the aminoglycoside-acetyltransferase (AAC) enzymes AAC(3), AAC(2') and AAC(6') by high-pressure liquid chromatographic characterization of their products of reaction with tobramycin or sisomicin is described. Conditions are given for the chromatography of kanamycin A, netilmicin, neomycin, and apramycin, and their products of reaction, if any, with the three AAC enzymes are listed.