Open AccessPhosphorylation of four acyclic guanosine analogs by herpes simplex virus type 2 thymidine kinase
Author(s) -
AnnaKarin Larsson,
Tao Pan
Publication year - 1984
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.25.4.524
Subject(s) - thymidine kinase , guanosine , guanine , thymidine , herpes simplex virus , phosphorylation , kinase , biology , nucleotide salvage , enzyme , biochemistry , chemistry , microbiology and biotechnology , virology , nucleotide , virus , dna , gene
Four acyclic guanosine analogs, the (R) and (S) enantiomers of 9-(3,4-dihydroxybutyl)guanine, 9-(4-hydroxybutyl)guanine, and acyclovir were compared in enzyme kinetic experiments, using purified herpes simplex virus type 2 thymidine kinase. All four analogs showed competitive patterns of inhibition in the phosphorylation of thymidine by the viral thymidine kinase, but different affinities and relative rates of phosphorylation were observed.