Open AccessHigh-pressure liquid chromatographic analysis of ceftazidime in serum and urine
Author(s) -
J. Steven Leeder,
Michael Spino,
Angelo Tesoro,
Stuart MacLeod
Publication year - 1983
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.24.5.720
Subject(s) - ceftazidime , chromatography , cephalosporin , urine , pharmacokinetics , chemistry , antibacterial agent , cephalosporin antibiotic , quantitative analysis (chemistry) , high performance liquid chromatography , antibiotics , pharmacology , medicine , biology , bacteria , biochemistry , genetics , pseudomonas aeruginosa
A rapid and sensitive high-pressure liquid chromatographic procedure was developed for quantitative analysis of a new semisynthetic cephalosporin, ceftazidime, in serum and urine. A good linear relationship was established between peak height and the amount of ceftazidime injected over a concentration range of 1.9 to 30 micrograms/ml. Recovery was approximately 90% at concentrations of 3, 15, and 30 micrograms/ml. The method is specific for ceftazidime, with no interference noted from 11 other beta-lactam antibiotics tested. The assay is accurate, reproducible, and useful for pharmacokinetic studies in humans as demonstrated in two subjects.