Open AccessHigh-pressure liquid chromatographic assay of ceftizoxime with an anion-exchange extraction technique
Author(s) -
Claudine E. Fasching,
Lance R. Peterson,
K M Bettin,
Dale N. Gerding
Publication year - 1982
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.22.2.336
Subject(s) - chromatography , ceftizoxime , chemistry , extraction (chemistry) , high performance liquid chromatography , coefficient of variation , ion exchange , ion chromatography , correlation coefficient , bioassay , ion , biology , mathematics , cefotaxime , biochemistry , statistics , organic chemistry , genetics , antibiotics
An anion-exchange extraction method was used in conjunction with high-pressure liquid chromatography for assay of ceftizoxime in 181 serum samples. Comparison of this method with bioassay gave a linear regression line described by Y = 1.11 + 0.98 X, with a correlation coefficient of 0.984. The anion-exchange extraction method is a fast, reliable method of preparing serum samples containing ceftizoxime for assay by liquid chromatography.