Open AccessVariables in demonstrating methicillin tolerance in Staphylococcus aureus strains
Author(s) -
Kouichi Ishida,
Phyllis A. Guze,
George M. Kalmanson,
Keith Albrandt,
Lucien B. Guze
Publication year - 1982
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.21.4.688
Subject(s) - staphylococcus aureus , microbiology and biotechnology , antibiotics , biology , strain (injury) , multidrug tolerance , staphylococcus , bacteria , genetics , biofilm , anatomy
Certain technical considerations which affected the status of methicillin tolerance in Staphylococcus aureus strains were studied. Methods which consistently demonstrated tolerance or intolerance of a given strain were avoidance of inoculum splashing, use of stationary-phase inoculum, 24-h tube incubation, and minimization of antibiotic carry-over. These studies suggested a need for the establishment of a standardized reference for the determination of tolerance.