Nuclear magnetic resonance spectrometric assay of beta-lactamase | Zendy

Mari Kono | Zendy; Koji Ohara | Zendy; Yoko Shiomi | Zendy

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Nuclear magnetic resonance spectrometric assay of beta-lactamase

Author(s) -

Mari Kono,

Koji Ohara,

Yoko Shiomi

Publication year - 1980

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.17.1.16

Subject(s) - cephalosporin , chemistry , nuclear magnetic resonance , beta lactamase , beta (programming language) , deuterium , nuclear magnetic resonance spectroscopy , antibiotics , biochemistry , physics , stereochemistry , nuclear physics , escherichia coli , computer science , gene , programming language

Beta-Lactam antibiotics and the crude enzyme were mixed in deuterium oxide and placed in a nuclear magnetic resonance tube. The change of the nuclear magnetic resonance spectrum during the enzymatic reaction was then analyzed to determine beta-lactamase activity. By using beta-lactam antibiotics such as penicillins, cephalosporins, and cephamycins as substrates, a comparison of the beta-lactamase activities was made between the nuclear magnetic resonance spectrometric assay and the iodometric assay. There was a close correlation between these two methods.

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