Open AccessNuclear magnetic resonance spectrometric assay of beta-lactamase
Author(s) -
Megumi Kono,
Koji Ohara,
Yoko Shiomi
Publication year - 1980
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.17.1.16
Subject(s) - cephalosporin , chemistry , nuclear magnetic resonance , beta lactamase , beta (programming language) , deuterium , nuclear magnetic resonance spectroscopy , antibiotics , biochemistry , physics , stereochemistry , nuclear physics , escherichia coli , computer science , gene , programming language
Beta-Lactam antibiotics and the crude enzyme were mixed in deuterium oxide and placed in a nuclear magnetic resonance tube. The change of the nuclear magnetic resonance spectrum during the enzymatic reaction was then analyzed to determine beta-lactamase activity. By using beta-lactam antibiotics such as penicillins, cephalosporins, and cephamycins as substrates, a comparison of the beta-lactamase activities was made between the nuclear magnetic resonance spectrometric assay and the iodometric assay. There was a close correlation between these two methods.